Home Biology Biotechnology Principles and Processes NCERT Examplar The most important feature in a plasmid to s…
Biology Biotechnology Principles and Processes NCERT Examplar Single Correct MCQ
Published on: August 21, 2026

The most important feature in a plasmid to serve as a vector in gene cloning experiment is:

A
Origin of replication
B
Presence of a selectable marker
C
Presence of sites for restriction endonuclease
D
Its size

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Verified by Experts
The correct answer is:
A

All of the given features are important to facilitate cloning into a vector but out of them, origin of replication (ori) is the most important one.
This is due to the following reasons:
(i) Ori is a DNA sequence that is responsible for initiating replication. Any piece of DNA when linked to this sequence can replicate within the host cells.
(ii) Ori also controls the copy numbers of the linked DNA.

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